Journal: bioRxiv

Article Title: Identification of targetable epigenetic vulnerabilities in uveal melanoma

doi: 10.1101/2024.10.11.617464

Figure Lengend Snippet: (A) Viability of the three UM cell lines following treatment with 932 epigenetic modulators at a concentration of 1 μM (n = 2). Hit cut-offs (dashed lines) were determined as the mean of the negative controls minus three standard deviations. (B) Radar plot showing the average percentage of cell death for the three cell lines treated with 932 compounds. (C) Pie charts of the molecular activities of all screened compounds (left) and the hits identified (right). (D) Dose-response experiments for the identified compounds (10 concentrations, n = 4 per concentration per cell line). (E) LogIC 50 values of the top-hit compounds for each cell line. Error bars represent 95% confidence interval. (F) LogIC 50 of BAP1 mutant cell lines (MP46 and MP38) plotted on the X-axis against the LogIC 50 of the BAP1 WT cell line (MP41) on the Y-axis.

Article Snippet: UM (MP41, MP46, and MP38) cell line stocks were obtained from the American Type Tissue Collection (ATCC).

Techniques: Concentration Assay, Mutagenesis

Journal: bioRxiv

Article Title: Identification of targetable epigenetic vulnerabilities in uveal melanoma

doi: 10.1101/2024.10.11.617464

Figure Lengend Snippet: (A) Concentration-response curves of MP41 and MP38 cells treated with the PARP inhibitor talazoparib. (B) Concentration-response curves of MP41 and MP38 cells treated with HDAC3 and HDAC8 inhibitors. N = 4 for each concentration.

Article Snippet: UM (MP41, MP46, and MP38) cell line stocks were obtained from the American Type Tissue Collection (ATCC).

Techniques: Concentration Assay